Moscow | Scientists have found how to control the behaviour of heart muscle cells using laser radiation, an advance that may lead to better understanding of the heart’s mechanisms and help treat arrhythmia or irregular heartbeat.
Right now this result may be very useful for clinical studies of the mechanisms of the heart, and in the future we could potentially stop attacks of arrhythmia in patients at the touch of a button, said Konstantin Agladze, head of Moscow Institute of Physics and Technology (MIPT)’s Laboratory of the Biophysics of Excitable Systems. Functional disorders in the heart muscles, particularly arrhythmia (an irregular heartbeat), are among the most common cardiac pathologies.
One in eight deaths in the world is caused by acute arrhythmia, researchers said. In order to study this type of heart disorder, it is important to be able to create arrhythmia in vitro, which is what azoTAB (azobenzene trimethylammonium bromide – a modified version of azobenzene) is used for.
An azoTAB molecule can exist in two states, switching between them under the influence of radiation. Agladze and his colleagues taught the azoTAB molecules to control cardiomyocytes so that one configuration did not prevent voluntary contractions (passive), and the other (active) deactivated contractions.
Using a device similar to a projector, but with a laser instead of a lamp, the scientists created at each point the required concentration of the active form of azoTAB. This enabled them to control the cardiomyocytes in each specific point of the heart. However, the precise mechanism of action of azoTAB on the cells remained unclear.
The scientists have now been able to explain how the different forms of azoTAB affect cardiomyocytes. Ion channels are used to transfer commands from one cell to another; they act as gates allowing ions to pass through cell membranes. In cardiomyocytes there are various types of channels capable of allowing potassium, sodium or calcium ions to pass.
The scientists conducted an experiment on heart muscle cells that were placed in a solution of azoTAB in two different concentrations. They were then exposed to light of different wavelengths in the range of near-UV light. When each of the channels was examined, the two others were deactivated using inhibitor substances and cardiomyocytes were isolated from one another.
It was found that after three minutes of exposure to the active form of azoTAB, the current through the calcium and sodium channels reduced by more than two times, and in the potassium channel it increased one and a half times. After the azoTAB was removed by washing the cells, the function of ion channels quickly returned to its normal state.
The experiment showed that the effect of azoTAB on a cell is reversible. This will mean that the results of the experiments will be able to be used in research and clinical practice, which could potentially lead to an effective treatment for arrhythmias.
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